EONS LEARNING

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  • Classroom
    • Resources >
      • Study Skills
      • Periodic Table
      • Common Ion Sheet
      • The Scientific Method
      • Doing Background Research
    • Introduction and Course Philosophy
    • Unit 1: Beginning Chemistry >
      • Lesson 1: The Atom >
        • What is an Atom?
        • The Structure of Atoms
        • The Periodic Table
        • Modeling Atoms
      • Lesson 2: Chemical Bonding >
        • Why Do Atoms Form Bonds?
        • Ionic Bonding
        • Covalent Bonding
        • Intermolecular Attraction
      • Lesson 3: Chemical Nomenclature >
        • Octet Rule
        • Ionic Compounds
        • Covalent Molecules
      • Lesson 4: Molecular Molecules >
        • Modeling Ionic Compounds
        • Modeling Covalent Molecules
      • Lesson 5: States of Matter >
        • States of Matter
        • Phase Changes
      • Lesson 6: Density >
        • What is Density?
        • Calculating Density
      • Lesson 7: Thermodynamics >
        • Temperature
        • Heat
        • Gas Laws
      • Lesson 8: Solution Chemistry >
        • Diffusion
        • Solutions and Molarity
        • Semi-Permeable Membranes
      • Lesson 9: Thermal Expansion >
        • Thermal Expansion
    • Unit 2: Earth Science >
      • Lesson 10: Earth at a Glance >
        • Perspective
        • Maps
      • Lesson 11: Layers of the Earth >
        • Layers of the Earth
      • Lesson 12: Plate Tectonics >
        • Plate Tectonics
      • Lesson 13: Rocks and Minerals >
        • Rocks and Minerals
      • Lesson 14: Particle Sorting >
        • Differentiation
        • Deposition of Sediment
      • Lesson 15: The Atmosphere >
        • Composition of the Atmosphere
        • Layers of the Atmosphere
        • Change Over Time
        • Atmospheric Disruption
    • Unit 3: The Cell >
      • Lesson 16: Life >
        • What is Life?
        • Structural Hierarchy of Living Things
      • Lesson 17: Biochemistry >
        • Intro to Biochemistry
        • Water
        • Micromolecules
        • Energy, Carbohydrates, Lipids
        • Protein and Nucleic Acid
      • Lesson 18: Cells >
        • What are Cells?
        • Microscopy
        • Plant and Animal Cells
      • Lesson 19: Membrane Transport >
        • A Special Environment
        • The Structure of Membranes
        • Membrane Transport
      • Lesson 20: Energy and Cell Respiration >
        • Energy in Biology
        • Energy Diagrams
        • Glycolysis and Anaerobic Respiration
        • Aerobic Cellular Respiration
      • Lesson 21: Photosynthesis >
        • Plants Get Energy From The Sun
        • Photosynthesis Process
        • Energy, Ecosystems, and the Environment
    • Unit 4: Anatomy and Physiology >
      • Lesson 22: The Human Body >
        • What Are Bodies Made Of?
        • What Do Bodies Do?
      • Lesson 23: The Nervous System >
        • The Nervous System
        • Neuronal Communication
        • The Central Nervous System
      • Lesson 24: The Endocrine System >
        • The Endocrine System
        • Hormones
        • Hormones, Puberty, and Reproduction
      • Lesson 25: The Integumentary System >
        • The Integumentary System
      • Lesson 26: The Musculoskeletal System >
        • The Skeletal System
        • The Muscular System
        • Anatomy Of The Musculoskeletal System
      • Lesson 27: The Cardiovascular System >
        • Blood and Blood Vessels
        • The Heart
      • Lesson 28: The Respiratory System >
        • The Respiratory System
      • Lesson 29: The Digestive System >
        • The Digestive System
        • Nutrition
      • Lesson 30: The Excretory System >
        • The Excretory System
      • Lesson 31: The Immune System >
        • Disease and Infection
        • Immunity
    • Units 5-6 Coming Soon
  • Workbench
    • Unit 1 >
      • EIS >
        • Lesson 1: Atoms
        • Lesson 2: Chemical Bonding
        • Lesson 3: Chemical Nomenclature
        • Lesson 4: Molecular Models
        • Lesson 5: States of Matter
        • Lesson 6: Density
        • Lesson 7: Thermodynamics
        • Lesson 8: Solution Chemistry
        • Lesson 9: Thermal Expansion
      • Unit 1: Project
      • Unit 1: Exam Review
      • Unit 1: Exam
    • Unit 2 >
      • EIS >
        • Lesson 10: Earth at a Glance
        • Lesson 11: Layers of the Earth
        • Lesson 12: Plate Tectonics
        • Lesson 13: Rocks and Minerals
        • Lesson 14: Particle Sorting
        • Lesson 15: The Atmosphere
      • Unit 2: Project
      • Unit 2: Exam Review
      • Unit 2: Exam
    • Unit 3 >
      • EIS >
        • Lesson 16: Life
        • Lesson 17: Biochemsitry
        • Lesson 18: Cells
        • Lesson 19: Membrane Transport
        • Lesson 20: Energy and Cell Respiration
        • Lesson 21: Photosynthesis
      • Unit 3: Project
      • Unit 3: Exam Review
      • Unit 3: Exam
    • Unit 4 >
      • EIS >
        • 22: The Human Body
        • 23: The Nervous System
        • 24: The Endocrine System
        • 25: The Integumentary System
        • 26: The Musculoskeletal System
        • 27: The Cardiovascular System
        • 28: The Respiratory System
        • 29: The Digestive System
        • 30: The Excretory System
        • 31: The Immune System
      • Unit 4 Project
      • Unit 4 Exam Review
      • Unit 4 Exam
    • Units 5-6 Coming Soon
  • Donate
  • Home
  • About Us
  • Classroom
    • Resources >
      • Study Skills
      • Periodic Table
      • Common Ion Sheet
      • The Scientific Method
      • Doing Background Research
    • Introduction and Course Philosophy
    • Unit 1: Beginning Chemistry >
      • Lesson 1: The Atom >
        • What is an Atom?
        • The Structure of Atoms
        • The Periodic Table
        • Modeling Atoms
      • Lesson 2: Chemical Bonding >
        • Why Do Atoms Form Bonds?
        • Ionic Bonding
        • Covalent Bonding
        • Intermolecular Attraction
      • Lesson 3: Chemical Nomenclature >
        • Octet Rule
        • Ionic Compounds
        • Covalent Molecules
      • Lesson 4: Molecular Molecules >
        • Modeling Ionic Compounds
        • Modeling Covalent Molecules
      • Lesson 5: States of Matter >
        • States of Matter
        • Phase Changes
      • Lesson 6: Density >
        • What is Density?
        • Calculating Density
      • Lesson 7: Thermodynamics >
        • Temperature
        • Heat
        • Gas Laws
      • Lesson 8: Solution Chemistry >
        • Diffusion
        • Solutions and Molarity
        • Semi-Permeable Membranes
      • Lesson 9: Thermal Expansion >
        • Thermal Expansion
    • Unit 2: Earth Science >
      • Lesson 10: Earth at a Glance >
        • Perspective
        • Maps
      • Lesson 11: Layers of the Earth >
        • Layers of the Earth
      • Lesson 12: Plate Tectonics >
        • Plate Tectonics
      • Lesson 13: Rocks and Minerals >
        • Rocks and Minerals
      • Lesson 14: Particle Sorting >
        • Differentiation
        • Deposition of Sediment
      • Lesson 15: The Atmosphere >
        • Composition of the Atmosphere
        • Layers of the Atmosphere
        • Change Over Time
        • Atmospheric Disruption
    • Unit 3: The Cell >
      • Lesson 16: Life >
        • What is Life?
        • Structural Hierarchy of Living Things
      • Lesson 17: Biochemistry >
        • Intro to Biochemistry
        • Water
        • Micromolecules
        • Energy, Carbohydrates, Lipids
        • Protein and Nucleic Acid
      • Lesson 18: Cells >
        • What are Cells?
        • Microscopy
        • Plant and Animal Cells
      • Lesson 19: Membrane Transport >
        • A Special Environment
        • The Structure of Membranes
        • Membrane Transport
      • Lesson 20: Energy and Cell Respiration >
        • Energy in Biology
        • Energy Diagrams
        • Glycolysis and Anaerobic Respiration
        • Aerobic Cellular Respiration
      • Lesson 21: Photosynthesis >
        • Plants Get Energy From The Sun
        • Photosynthesis Process
        • Energy, Ecosystems, and the Environment
    • Unit 4: Anatomy and Physiology >
      • Lesson 22: The Human Body >
        • What Are Bodies Made Of?
        • What Do Bodies Do?
      • Lesson 23: The Nervous System >
        • The Nervous System
        • Neuronal Communication
        • The Central Nervous System
      • Lesson 24: The Endocrine System >
        • The Endocrine System
        • Hormones
        • Hormones, Puberty, and Reproduction
      • Lesson 25: The Integumentary System >
        • The Integumentary System
      • Lesson 26: The Musculoskeletal System >
        • The Skeletal System
        • The Muscular System
        • Anatomy Of The Musculoskeletal System
      • Lesson 27: The Cardiovascular System >
        • Blood and Blood Vessels
        • The Heart
      • Lesson 28: The Respiratory System >
        • The Respiratory System
      • Lesson 29: The Digestive System >
        • The Digestive System
        • Nutrition
      • Lesson 30: The Excretory System >
        • The Excretory System
      • Lesson 31: The Immune System >
        • Disease and Infection
        • Immunity
    • Units 5-6 Coming Soon
  • Workbench
    • Unit 1 >
      • EIS >
        • Lesson 1: Atoms
        • Lesson 2: Chemical Bonding
        • Lesson 3: Chemical Nomenclature
        • Lesson 4: Molecular Models
        • Lesson 5: States of Matter
        • Lesson 6: Density
        • Lesson 7: Thermodynamics
        • Lesson 8: Solution Chemistry
        • Lesson 9: Thermal Expansion
      • Unit 1: Project
      • Unit 1: Exam Review
      • Unit 1: Exam
    • Unit 2 >
      • EIS >
        • Lesson 10: Earth at a Glance
        • Lesson 11: Layers of the Earth
        • Lesson 12: Plate Tectonics
        • Lesson 13: Rocks and Minerals
        • Lesson 14: Particle Sorting
        • Lesson 15: The Atmosphere
      • Unit 2: Project
      • Unit 2: Exam Review
      • Unit 2: Exam
    • Unit 3 >
      • EIS >
        • Lesson 16: Life
        • Lesson 17: Biochemsitry
        • Lesson 18: Cells
        • Lesson 19: Membrane Transport
        • Lesson 20: Energy and Cell Respiration
        • Lesson 21: Photosynthesis
      • Unit 3: Project
      • Unit 3: Exam Review
      • Unit 3: Exam
    • Unit 4 >
      • EIS >
        • 22: The Human Body
        • 23: The Nervous System
        • 24: The Endocrine System
        • 25: The Integumentary System
        • 26: The Musculoskeletal System
        • 27: The Cardiovascular System
        • 28: The Respiratory System
        • 29: The Digestive System
        • 30: The Excretory System
        • 31: The Immune System
      • Unit 4 Project
      • Unit 4 Exam Review
      • Unit 4 Exam
    • Units 5-6 Coming Soon
  • Donate

Microscopy

Micro-what?

You may have heard the word microscope, which is that thing that scientists use when they want to look science-y. (And when they want to look at really small stuff. Which, depending on the scientist, might be all day, every day). It’s basically a really fancy, powerful magnifying glass. Microscopy is just the use of a microscope.

To talk about cells, we really have to mention microscopes. That’s because cells are microscopic, so we need a microscope to see them. Most things we know about cells we know, at least in part, because of microscopy.

Types of Microscopes

Regular light microscopes—the ones you’re probably most familiar with—can allow us to see up to about 400 times the size of an object. They might take an image like this:
Picture
Blood under a light microscope
There are also other kinds of microscopes, like fluorescence microscopes, which might take an image like this:
Picture
Blood under a fluorescence microscope
Another type of microscope is the electron microscope, which can magnify up to 500,000 times. They might take an image like this:
Picture
Blood under an electron microscope
There are also many other types of microscopes out there. The only one you need to know the details of for this class is the standard light microscope.

Standard Light Microscope

This is a standard light microscope, like one that you might find in just about any biology classroom or lab (including the labs that real biologists use) around the world:
Picture
The key parts of this are the eyepiece (the part you look through with your eyes, so that you can actually see what you’re looking at--it also magnifies somewhat), the light source (which makes things bright, so that you can see), and this part...:
Picture
...which is called the objective. This is where most of the magnification happens.

Most microscopes, including this one, have multiple objectives in different magnifications (usually 4x to 40x, for a total magnification, when you factor in the eyepiece, of 40x to 400x). Larger objectives give more magnification, so that you can see the smallest stuff. Having multiple objectives gives the viewer control over how much he or she wants to zoom in. You can look at stuff in “wide view” (for example, using a 4x objective) to get a more complete picture of what something looks like, and “super-close up” (for example, using a 40x objective) to get in super close on the details.

The logic behind which one to use is basically the same as how you would decide whether to take a picture of someone where you can see their whole body versus one that’s zoomed in really close on their nose. Both could be interesting, but they have very different purposes. If, for example, you’re trying to see if a person has freckles, the really zoomed in one might be more helpful.

Light microscopes are pretty easy to use, once you know how to do it. As such, watching someone use a light microscope can be pretty boring. If you have the ability to use a microscope in person, it can be a lot of fun to look at all sorts of different everyday objects just to see what you can see, and to learn about the microscopic structure of these everyday objects. STEM (Science, Technology, Engineering, and Mathematics) fairs and camps are a great place to do this. Schools and community centers also often have microscopes, and some relatively cheap ones designed for kids to explore with can be purchased in stores or online.

This video gives a great overview of using a microscope:
​


​Neat, right?

Looking At Stuff Under A Microscope

So, what does stuff look like under a microscope? It’s a little bit tricky to show you on a computer screen exactly what something looks like at a certain magnification under a microscope, because pictures change size depending on the size of your screen and how much you are zoomed in. But here are some cool pictures of stuff under microscopes, just because it’s interesting. Note that the colors come from how we stain stuff to see it better; really, this close up, most stuff is just various shades of clear:
Picture
Picture
Picture
Blood
Picture
Leaf
Onion
Picture
Staphylococcus aureus, a common type of bacteria (Staph infection)
Skin
Picture
Escheria coli, another common type of bacteria (E. coli)

​Feel free to search the web (using all standard precautions for searching the web, of course) for pictures of whatever interesting thing strikes your fancy under a microscope.


Just for fun, here are some pictures of stuff under a scanning electron microscope, which is a super powerful kind of microscope that lets you see a lot of the really cool close-up 3D details of stuff. The colors are added in post-processing for artistic effect:
Picture
Picture
                                                          Flu virus 
                 Cancer Cell Dividing 

Summary

You should understand:
  • How to use a light microscope.
That an objective of a light microscope is where most of the magnification happens, and that most microscopes have multiple objectives to adjust magnification.

Learning Activity

Picture
Next: plant and animal cells

Content contributors: Emma Moulton and Emily Zhang
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